Abstracts:

TOTAL LUMINESCENCE SPECTRA OFPyrodinium bahamense var compressum

Ma. Christina Z. Floresca1, Susan Sta. Ana1, Edgar Vallar1, Maria Cecilia Galvez2, and Rhodora Azanza3

Departments of 1Chemistry and 2Physics, De La Salle University, 2401 Taft Ave. Manila, Philippines 1004,and 3Marine Science Institute, University of the Philippines, Diliman, Quezon City, Philippines, 1100


Pyrodinium bahamense var compressum cultures and in situ samples collected from various parts of Manila Bay were analyzed by total luminescence spectroscopy in the ultraviolet and visible region. Synchronous fluorescence intensities from 200 to 800 nm were collected over various excitation wavelengths from 200 to 800 nm by using a Shimadzu RF-5301 double monochromator spectrofluorimeter. Data was plotted by using Surfer ú Surface Mapping System (Golden Software) on an IBM PC. Emission peaks were observed at 421-470 nm and at around 654 nm. Thus, a fingerprint of the organism was obtained from measured spectra. This data is to be the basis for development of a fluorescence LIDAR detection system for monitoring red tide blooms.

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