Brevetoxins (PbTxs), a class of marine lipid-soluble polyether compounds produced by the ichtyotoxic dinoflagellate Gymnodinium breve (Ptychodiscus brevis), are toxic for human both after direct exposure (sea-aerosol) or ingestion of toxic shellfish. Although numerous techniques for detection and quantitation of PbTxs are available or under development, including animal bioassays (mouse andfish), HPLC, molecular pharmacological assays using Voltage Sensitive Sodium Channel (VSSC) preparations, only the mouse bioassay is currently used to monitor the shellfish toxicity. Immunoassays (RIA and ELISA) using polyclonal antibodies have been documented since the pioneer study of Baden et al. (1984). However up to now monoclonal antibody (MAb) to PbTxs has not been produced. This work presents the first evidence of an unlimited source of standard material to design systems for detection, quantification, concentration and/or labeling of brevetoxins. Production technique and the full characterization of this Mab raised against type-2 brevetoxins are also described.