James Horne

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James horne

James Horne

Research Fellow - Nuclear Magnetic Resonance

Room 338, Chemistry Building, Sandy Bay Campus

+61 3 6226 7821 (phone)

+61 3 6226 2494 (fax)

Additional Information

In recent years James has had various roles including: Research assistant with Pfizer Central Research, Sandwich UK and Biochemistry, University of Cambridge. Cloning, expression and purification of isotopically labelled proteins for NMR Postdoctoral researcher, Biochemistry, University of Cambridge with Professor RN Perham. NMR structure determination and analysis of excised domains from the multienzyme complexes of pyruvate dehydrogenase and glutathione reductase. Postdoctoral researcher, Biochemistry, University of Cambridge with Professor RN Perham. NMR structure determination and analysis of excised domains from the multienzyme complexes of pyruvate dehydrogenase and glutathione reductase Postdoctoral researcher, St Vincents Institute of Medical Research with Dr Bostjan Kobe and Professor Bruce Kemp. Computational analysis of peptide substrate binding to protein kinases and NMR investigation of autoregulatory sequence of phenylalanine hydroxylase Postdoctoral research fellow, Department of Medicinal Chemistry, Victorian College of Pharmacy, Monash University. Implementation of methods for triple resonance NMR investigation of large proteins. Structural determination and dynamical characterisation of a bacterial DsbA oxidireductase enzymes. Method development and conduct of small molecule fragment library screening against protein targets, including DsbA enzymes. A variety of other structural and physical characterisations across projects involved in lipid transport and neurodegenerative disease states.

View more on Dr James Horne in WARP

Research Fields

  • Structural Biology (incl. Macromolecular Modelling) (060112)
  • Pharmaceutical Sciences (111504)
  • Industrial Chemistry (039903)
  • Characterisation of Biological Macromolecules (030403)
  • Plant Physiology (060705)
  • Natural Products Chemistry (030502)
  • Cell Metabolism (060104)
  • Transition Metal Chemistry (030207)
  • Cardiology (incl. Cardiovascular Diseases) (110201)
  • Medical Bacteriology (110801)
  • Medical Biochemistry and Metabolomics (110199)
  • Biologically Active Molecules (030401)
  • Primary Health Care (111717)
  • Fish Pests and Diseases (070404)

Research Impact

  • Expanding Knowledge in the Biological Sciences (970106)
  • Expanding Knowledge in the Chemical Sciences (970103)
  • Infectious Diseases (920109)
  • Cardiovascular System and Diseases (920103)
  • Expanding Knowledge in the Medical and Health Sciences (970111)
  • Public Health (excl. Specific Population Health) (920499)
  • Neurodegenerative Disorders Related to Ageing (920112)
  • Paper Products (incl. Coated Paper) (860501)
  • Aquaculture Fin Fish (excl. Tuna) (830102)
  • Pulp and Paper (860503)
  • Human Pharmaceutical Products (860899)
  • Expanding Knowledge in the Environmental Sciences (970105)


Total publications


Journal Article

(16 outputs)
2015Nikolic SB, Edwards LM, Karpievitch YV, Wilson R, Horne J, et al., 'Serum metabolic profile predicts adverse central haemodynamics in patients with type 2 diabetes mellitus', Acta Diabetologica, (September) pp. 1-9. ISSN 1432-5233 (2015) [Refereed Article]

DOI: 10.1007/s00592-015-0802-4 [eCite] [Details]

Co-authors: Nikolic SB; Karpievitch YV; Wilson R; Adams MJ; Sharman JE


2015Shastri MD, Stewart N, Horne J, Zaidi STR, Sohal SS, et al., 'Non-anticoagulant fractions of enoxaparin suppress inflammatory cytokine release from peripheral blood mononuclear cells of allergic asthmatic individuals', PLoS One, 10, (6) Article e0128803. ISSN 1932-6203 (2015) [Refereed Article]

DOI: 10.1371/journal.pone.0128803 [eCite] [Details]

Citations: Scopus - 1

Co-authors: Shastri MD; Stewart N; Zaidi STR; Sohal SS; Peterson GM; Korner H; Gueven N; Patel RP


2015Shastri MD, Stewart N, Horne J, Peterson GM, Gueven N, et al., 'In-vitro suppression of IL-6 and IL-8 release from human pulmonary epithelial cells by non-anticoagulant fraction of enoxaparin', PLoS One, 10, (5) Article e0126763. ISSN 1932-6203 (2015) [Refereed Article]

DOI: 10.1371/journal.pone.0126763 [eCite] [Details]

Co-authors: Shastri MD; Stewart N; Peterson GM; Gueven N; Sohal SS; Patel RP


2014Adams LA, Sharma P, Mohanty B, Ilyichova OV, Mulcair MD, et al., 'Application of fragment-based screening to the design of inhibitors of Escherichia coli DsbA', Angewandte Chemie, 54, (7) pp. 2179-2184. ISSN 1433-7851 (2014) [Refereed Article]

DOI: 10.1002/anie.201410341 [eCite] [Details]

Citations: Web of Science - 1


2014Deans RM, Gardiner MG, Horne J, Hung AC, Hyland CJT, et al., 'Isolation and characterization of 1β-Acetoxypolygodial from Tasmannia Lanceolata', Asian Journal of Organic Chemistry, 3, (11) pp. 1193-1196. ISSN 2193-5807 (2014) [Refereed Article]

DOI: 10.1002/ajoc.201402105 [eCite] [Details]

Co-authors: Deans RM; Gardiner MG; Hung AC; Hyland CJT; Just J; Smith JA


2014Musgrove RE, Horne H, Wilson R, King AE, Edwards LM, et al., 'The metabolomics of alpha-synuclein (SNCA) gene deletion and mutation in mouse brain', Metabolomics, 10, (1) pp. 114-122. ISSN 1573-3882 (2014) [Refereed Article]

DOI: 10.1007/s11306-013-0561-6 [eCite] [Details]

Citations: Scopus - 2Web of Science - 2

Co-authors: Musgrove RE; Wilson R; King AE; Edwards LM; Dickson TC


2012Edwards LM, Lawler NG, Nikolic SB, Peters JM, Horne J, et al., 'Metabolomics reveals increased isoleukotoxin diol (12,13-DHOME) in human plasma after acute Intralipid infusion', Journal of Lipid Research, 53, (9) pp. 1979-1986. ISSN 0022-2275 (2012) [Refereed Article]

DOI: 10.1194/jlr.P027706 [eCite] [Details]

Citations: Scopus - 10Web of Science - 7

Co-authors: Lawler NG; Nikolic SB; Peters JM; Wilson R; Davies NW; Sharman JE


2012Tivendale ND, Davidson SE, Davies NW, Smith JA, Dalmais M, et al., 'Biosynthesis of the halogenated auxin, 4-chloroindole-3-acetic acid', Plant Physiology, 159, (3) pp. 1055-1063. ISSN 0032-0889 (2012) [Refereed Article]

DOI: 10.1104/pp.112.198457 [eCite] [Details]

Citations: Scopus - 19Web of Science - 17

Co-authors: Tivendale ND; Davidson SE; Davies NW; Smith JA; Quittenden LJ; Bala RK; Reid JB; Ross JJ


2011McLean D, Agarwal V, Stack K, Horne HJ, Richardson D, 'Synthesis of guar gum-graft-poly (acrylamide-co-diallyldimethylammonium chloride) and its application in the pulp and paper industry', BioResources, 6, (4) pp. 4168-4180. ISSN 1930-2126 (2011) [Refereed Article]

[eCite] [Details]

Citations: Scopus - 10Web of Science - 7

Co-authors: McLean D; Agarwal V; Stack K

2011Velkov T, Horne J, Scanlon MJ, Capuano B, Yuriev E, et al., 'Characterization of the N-Methyltransferase Activities of the Multifunctional Polypeptide Cyclosporin Synthetase ', Chemistry and Biology, 18, (4) pp. 464-475. ISSN 1074-5521 (2011) [Refereed Article]

DOI: 10.1016/j.chembiol.2011.01.017 [eCite] [Details]

Citations: Scopus - 3Web of Science - 3


2010McGuinness DS, Davies NW, Horne HJ, Ivanov I, 'Unraveling the mechanism of polymerisation with the Phillips catalyst', Organometallics, 29, (22) pp. 6111-6116. ISSN 0276-7333 (2010) [Refereed Article]

DOI: 10.1021/om100883n [eCite] [Details]

Citations: Scopus - 16Web of Science - 13

Co-authors: McGuinness DS; Davies NW


2009Chuang S, Velkov T, Horne HJ, Wielens J, Chalmers DK, et al., 'Probing the fibrate binding specificity of rat liver fatty acid binding protein', Journal of Medicinal Chemistry, 52, (17) pp. 5344-55. ISSN 0022-2623 (2009) [Refereed Article]

DOI: 10.1021/jm801349e5344-55 [eCite] [Details]

Citations: Scopus - 12Web of Science - 12


2009Paxman JJ, Borg NA, Horne HJ, Thompson PE, Chin Y, et al., 'The structure of the bacterial oxidoreductase enzyme DsbA in complex with a peptide reveals a basis for substrate specificity in the catalytic cycle of DsbA enzymes', Journal of Biological Chemistry, 284, (26) pp. 17835-17845. ISSN 0021-9258 (2009) [Refereed Article]

DOI: 10.1074/jbc.M109.011502 [eCite] [Details]

Citations: Scopus - 23Web of Science - 26


2008Robertson AJ, Horne HJ, Ellisdon AM, Thomas B, Scanlon MJ, et al., 'The structural impact of a polyglutamine tract is location dependent', Biophysical Journal, 95, (1) pp. 5922-5930 . ISSN 0006-3495 (2008) [Refereed Article]

DOI: 10.1529/biophysj.108.138487 [eCite] [Details]

Citations: Scopus - 19Web of Science - 15


2008Vivian JP, Scoullar J, Robertson AL, Bottomley SP, Horne HJ, et al., 'Structural and Biochemical Characterization of the Oxidoreductase NmDsbA3 from Neisseria meningitidis', Journal of Biological Chemistry, 283, (47) pp. 32452-32461. ISSN 1083-351X (2008) [Refereed Article]

DOI: 10.1074/jbc.M803990200 [eCite] [Details]

Citations: Scopus - 15Web of Science - 17


2007Velkov T, Horne HJ, Laguerre A, Jones E, Scanlon MJ, et al., 'Examination of the role of intestinal fatty acid-binding protein in drug absorption using a parallel artificial membrane permeability assay', Chemistry and Biology, 14, (4) pp. 453-465. ISSN 1074-5521 (2007) [Refereed Article]

DOI: 10.1016/j.chembiol.2007.03.009 [eCite] [Details]

Citations: Scopus - 19Web of Science - 16


Conference Publication

(1 outputs)
2010Rao RN, Stack KR, Lewis TW, Richardson D, Horne HJ, 'Using NMR and SEM, to study the behaviour of pitch', 64th Appita Annual Conference and Exhibition Proceedings, 18-21 April 2010, Melbourne, pp. 281-287. (2010) [Non Refereed Conference Paper]

[eCite] [Details]

Co-authors: Rao RN; Stack KR; Lewis TW

Grants & Funding

Funding Summary

Number of grants


Total funding



Chemika impurity characterisation - ICH guidelines (2014)$6,915
This project will identify degradation products that arise during routine quality control assays as part of compliance with ICH guidelines. Degradation products will be identified using a three step process of UPLC with low resolution MS and fractionation and purity testing, followed by high resolution MS (orbitrap), with subsequent NMR.
Chemika Pty ltd ($6,915)
Administered By
University of Tasmania
Research Team
Jacobson GA; Davies NW; Wilson RK; Horne HJ; Smith JA