Central Science Laboratory

Nuclear Magnetic Resonance Spectroscopy

The NMR facility at the CSL underwent substantial upgrade and expansion in 2013. The equipment comprises two Bruker Avance III HD spectrometers at 400 and 600 MHz respectively. Both are equipped for solution-state spectroscopy applications.

The 400 MHz spectrometer is a wide bore magnet with a 5mm BBFO broadband tuneable probe capable of observing nuclei across a wide range of frequencies. This two rf channel system has automatic tuning, matching and shim capability and is a fast, robust and highly usable instrument for routine analyses for Chemistry. The probe can operate over a wide temperature range from –80C to +120C to assist in characterisation of dynamic processes and to allow for monitoring of highly reactive species. This is the go-to instrument for observation of less common NMR nuclei.

The 600 MHz spectrometer is a narrow bore, actively shielded magnet, four channel rf system with a cryocooled 5mm TCI probe tuneable to 1H/19F, 13C, 15N and 2H nuclei simultaneously. This allows for multinuclear, multidimensional analysis of large, complex, isotope-labelled biomolecules for the understanding of structure, dynamics and molecule-molecule interactions. The sensitivity increase from the cryogenic cooling of the probe electronics permits the characterisation of compounds in relatively low amounts, often from natural product extractions. The characterisation of complex mixtures is a common application for this system, particularly metabolomic investigation of biological tissues and extracts for medical, agricultural and other life sciences in a range of scenarios. This is often used in tandem with orthogonal techniques such as GC-MS and Orbitrap-MS to complement and extend coverage of biological profiling.

These systems are well-equipped to support the gamut of contemporary solution-state spectroscopy methods for application in the Chemical, Biological/Medical and material sciences.

For more information and to discuss potential project ideas please contact Dr James Horne.