Profiles
Anthony Cook

Anthony Cook
Associate Professor
Wicking Dementia Research & Education Centre
Room 417a , Medical Science 1 (MS1)
+61 3 6226 6964 (phone)
Dr Anthony Cook researches age-related neurodegenerative diseases such as Alzheimer’s disease and motor neurone disease. The unifying theme of his research is the study of human cell-based models.
Many researchers use animal models such as fruit flies or mice to investigate human diseases. ‘One of the advantages of studying human cells,’ explains Dr Cook, ‘is that they possess an inherent genetic variability between them that reflects human populations. Animal models of disease don’t capture this variation quite as well.’
‘At the Wicking Centre, I’ve been developing human cell-based models that complement animal-based research. The reason that we need these models is so we can see whether the findings from animal-based research are transferable to humans. If the same responses occur in human cells, then it’s a good sign that the animal model findings could be useful for developing potential therapies.’ Dr Cook notes that his research is a ‘key component of translating bench research to useful new therapies’.
Dr Cook’s laboratory uses induced pluripotent stem (iPS) cell technology. ‘It was developed in Japan,’ he says, ‘and has revolutionised health and medical research.’ Research using iPS cell technology begins with a live person – cells, typically skin or blood cells, are obtained from a donor using ethically approved protocols, and these are ‘reprogrammed’ in the laboratory. ‘This reprogramming creates a cell that we call an induced pluripotent stem cell, which is highly similar to an embryonic stem cell.’
iPS cell technology allows researchers to study brain cells from people living with neurodegenerative diseases. ‘Once we have the iPS cells, we mimic the normal developmental processes of a human cell by introducing biochemical cues in a certain order and timing, to turn them into the cell type we’re interested in. So, if we’re studying the brain, we can turn iPS cells into neurons that we can use to model disease processes.’ Dr Cook explains that iPS cell technology is so powerful because it makes it possible to study nerve cells from large numbers of people with different genetic risk profiles.
Another exciting aspect of his research, says Dr Cook, is another technological advance known as CRISPR/Cas gene editing. ‘We can take the iPS cells from people with gene variants that cause a disease and use CRISPR/Cas technology to correct the gene sequence to that found in people without the disease.’ This produces isogenic cell lines – a pair of cell lines that are genetically identical except for the disease-causing variant. ‘These can be turned into neurons or whatever cell type is needed, and we can study how the gene variant changes the function of those cells.’
Dr Cook entered the field by following opportunities that interested him, many of which had the potential to make a difference to human health. ‘It started with a little bit of serendipity. I enrolled in engineering and found that it wasn’t for me, so I went and did a science degree. Then, having never studied biology at high school, I did first year biochemistry, and remember clearly one of the lecturers saying we can make transgenic animals. This intrigued me and set in motion my whole undergraduate degree in which I studied as much molecular and cell biology as I could, and became involved in a research group through an undergrad lab placement in my summer holidays.
‘UTAS is a great place to be doing biomedical research,’ says Dr Cook. ‘We have a highly engaged population who are genuinely interested in what we do and who are willing to participate in our research. And because the Wicking Centre is part of the College of Health and Medicine, and we are in the one building with both the Menzies Institute for Medical Research and the School of Medicine, there are many opportunities for collaboration.’
Where might this research lead? ‘There is potential for iPS cell technology to provide a pathway to precision medicine,’ says Dr Cook. ‘For example, iPS cells make it possible to conduct drug discovery experiments using nerve cells from many people, to identify drugs that are effective, and to identify those cells that respond versus those that do not. Both the development of new treatments and the possibility of tailoring those treatments on an individual basis mean that iPS cell technology has the potential to improve health outcomes for a range of conditions.’
Dr Cook is a Senior Lecturer within the Wicking Dementia Research and Education Centre. He research interests lie in developing and utilising human cell-based models of neurodegenerative diseases to uncover molecular pathways that contribute to disease onset and progression.
Biography
Dr Cook completed his BSc and BSc (Hons, First Class) degrees in 1999 and 2000 respectively, at The University of Queensland (UQ), majoring in biochemistry for each. He subsequently undertook research studies towards the degree of Doctor of Philosophy in Assoc Prof Rick Sturm's laboratory within the Institute for Molecular Bioscience at UQ, and was awarded a PhD in the Field of Molecular Biology/Biochemistry in 2004. His thesis was awarded the Dean's commendation for Outstanding Research Higher Degree Theses 2004.
Since completion of his PhD, Dr Cook has been employed as a post-doctoral scientist at UQ, Griffith University (Queensland, Australia) and the University of Tasmania (UTas). From 2010 - June 2015, he was the employed as a Lecturer in Human Biological Science within the School of Health Sciences, and from July 2015 as a Senior Lecturer within the Wicking Dementia Research and Education Centre at The University of Tasmania, where he developed an extensive teaching portfolio that includes cell biology, biochemistry/ molecular biology, and molecular genetics at all undergraduate levels.
Career summary
Qualifications
- PhD, University of Queensland, Australia. 2004 Thesis: Models of Human Neural Crest Cell Differentiation in Vitro
- Graduate Certificate in University Learning and Teaching, University of Tasmania, Australia. 2015
- BSc Hons (1st Class), University of Queensland, Australia 2000 Thesis: Homeobox Gene Expression in Merkel Cell Carcinoma
- BSc, University of Queensland, Australia. 1999
Memberships
Professional practice
- Member, Association for Research in Vision and Ophthalmology
- Member, Australian Society for Stem Cell Research
- Member, Australian Society for Medical Research
- Member, Molecular and Experimental Pathology Society of Australia
Committee associations
- Member, Institutional Biosafety Committee, University of Tasmania (2011 - present)
- Co-ordinator, Undergraduate Research Opportunity Program (UROP), School of Health Sciences, University of Tasmania (2013-2014)
Other
- Convenor, Medical Research Week Gala Dinner, Australian Society for Medical Research (Tasmanian Branch), for 2011, 2012 and 2014.
Teaching
Cell biology, biochemistry, molecular biology, molecular genetics, dementia, neurobiology, neuroscience
Teaching expertise
Dr Cook has a teaching portfolio that includes design, delivery and assessment within Units encompassing cell biology, biochemistry and molecular biology, and molecular genetics at all undergraduate levels, and in flexible delivery modes (face-to-face and fully online). Furthermore, he has taught these topics to students undertaking health-related degrees in fields other than with biochemistry/molecular biology majors, including biomedical science (pathology), exercise science, and dementia studies. He is interested in pedagogical approaches to teaching and learning that are inquiry driven and which teach science as science is practiced.
Teaching responsibility
Dr Cook acts as co-ordinator for several Units within the Bachelor of Dementia Care, including Advanced Topics in Neurobiology of Dementia (CAD302).
Research Appointments
Dr Cook was a participant in the Theo Murphy High Flyers Think Tank 2015 on the topic the Stem Cell Revolution – lessons and imperatives for Australia, an event organised by the Australian Academy of Science.
View more on AssocProf Tony Cook in WARP
Expertise
- Modelling of human disease and phenotypic traits using donor-specific cell lines
- Mammalian cell culture techniques (including establishing primary cell cultures, and in maintenance and differentiation of stem cell cultures)
- Cell and molecular biology techniques
Research Themes
Dr Cook's research aligns to the University's Better Health research theme. His research interests include development of human cell-based models of disease that facilitate studying the underlying biology of disease onset or progression. These interests stem from solid doctoral and post-doctoral training in the development of human cell-based models to study the molecular and cellular biology of genetic traits and disease mechanisms (natural variation in pigmentation, and Parkinson's disease [PD], respectively). Since joining the University of Tasmania, Dr Cook has focused his research on human stem cell-based models of two age-related chronic neurodegenerative diseases: dementia, and the blinding eye disease glaucoma. By directly studying the biology of human cells from people with disease, Dr Cook's research will help to identify new possibilities for prevention or treatment, and which may ultimately translate to improved healthcare outcomes.
Collaboration
Dr Cook has active collaborations with other scientists within the Wicking Dementia Research and Education Centre, with the Faculty of Health at the University of Tasmania, with Tasmanian-based ophthalmologists, and nationally with stem cell researchers.
Current projects
- Stem cell derived models of age-related degenerative diseases:
- Dementia In this project, we are using donor-specific cells derived from participants from the Tasmanian Healthy Brain Project. Induced pluripotent stem cells (iPSCs) corresponding to each donor are matured into neurons in the laboratory for molecular charaterisation and functional assays. This work is collaboration involving a large number of researchers within the wicking Dementia Research and Education Centre, and other local and national scientists.
- Glaucoma In this project, we are investigating the cellular and molecular changes that occur within various cell types within the eye, including retinal ganglion cells, astrocytes, and trabecular meshwork cells, that contribute to the blinding disease glaucoma. This work is being done in collaboration with Assoc. Prof. Alex Hewitt (School of Medicine, UTas / Menzies) and Dr Alice Pebay (Centre for Eye Research Australia).
- Molecular analysis of clinical samples obtained from glaucoma patients during surgical treatment for advanced disease. This involves collection of aqueous humor for cytokine and growth factor profiling. This work is being done in collaboration with Dr Tze'Yo Toh, a glaucoma and cataract specialist at the Launceston Eye Institute.
Fields of Research
- Cellular nervous system (320902)
- Cancer genetics (321103)
- Cell development, proliferation and death (310102)
- Neurology and neuromuscular diseases (320905)
- Ophthalmology and optometry (321299)
- Ophthalmology (321201)
- Biochemistry and cell biology (310199)
- Cell metabolism (310103)
- Epigenetics (incl. genome methylation and epigenomics) (310504)
- Genetic immunology (310507)
- Signal transduction (310111)
- Gene and molecular therapy (320601)
- Vision science (321204)
- Animal physiology - systems (310910)
- Central nervous system (320903)
- Education assessment and evaluation (390402)
- Neurosciences (320999)
- Regenerative medicine (incl. stem cells) (320606)
- Nutritional science (321004)
- Nutrition and dietetics (321099)
- Genetics (310599)
- Nanotoxicology, health and safety (320605)
- Science, technology and engineering curriculum and pedagogy (390113)
- Genomics and transcriptomics (310204)
- Molecular medicine (340406)
- Cancer cell biology (321101)
- Medical biochemistry and metabolomics (320599)
- Gastroenterology and hepatology (320209)
- Animal physiology - cell (310909)
- Health services and systems (420399)
- Peripheral nervous system (320906)
- Health psychology (520304)
- Sensory systems (320907)
- Cell neurochemistry (310104)
- Biomechanical engineering (400303)
- Analytical chemistry (340199)
- Cellular immunology (320404)
- Separation science (340109)
- Genomics (310509)
- Animal immunology (310905)
- Cell physiology (320801)
- Other chemical sciences (349999)
Research Objectives
- Clinical health (200199)
- Treatment of human diseases and conditions (200105)
- Expanding knowledge in the health sciences (280112)
- Expanding knowledge in the biomedical and clinical sciences (280103)
- Expanding knowledge in the biological sciences (280102)
- Prevention of human diseases and conditions (200104)
- Other health (209999)
- Learner and learning (160199)
- Terrestrial biodiversity (180606)
- Behaviour and health (200401)
- Other education and training (169999)
- Public health (excl. specific population health) (200499)
- Diagnosis of human diseases and conditions (200101)
- Health status (incl. wellbeing) (200407)
- Expanding knowledge in the chemical sciences (280105)
- Aboriginal and Torres Strait Islander determinants of health (210301)
Publications
All of Dr Cook's papers have been published in peer-reviewed international journals that are well-regarded journals in their area of research. His publication record reflects his skills and experience in development of novel human cell culture models, and their use to model human diseases and phenotypic traits.
Total publications
55
Highlighted publications
(5 outputs)Year | Type | Citation | Altmetrics |
---|---|---|---|
2020 | Journal Article | Ooi L, Dottori M, Cook AL, Engel M, Gautam V, et al., 'If human brain organoids are the answer to understanding dementia, what are the questions?', The Neuroscientist, 26, (5-6) pp. 438-454. ISSN 1073-8584 (2020) [Refereed Article] DOI: 10.1177/1073858420912404 [eCite] [Details] Citations: Scopus - 8Web of Science - 7 Co-authors: King AE | |
2015 | Journal Article | Ashworth Briggs EL, Toh TY, Eri RD, Hewitt A, Cook AL, 'TIMP1, TIMP2, and TIMP4 are increased in aqueous humor from primary open angle glaucoma patients', Molecular Vision, 21 pp. 1162-1172. ISSN 1090-0535 (2015) [Refereed Article] PMID: 26539028 [eCite] [Details] Citations: Web of Science - 31 Co-authors: Ashworth Briggs EL; Eri RD; Hewitt A | |
2010 | Journal Article | Matigian N, Abrahamsen G, Sutharsan R, Cook AL, Vitale AM, et al., 'Disease-specific, neurosphere-derived cells as models for brain disorders', Disease models & mechanisms, 3, (11) pp. 785-798. ISSN 1754-8403 (2010) [Refereed Article] DOI: 10.1242/dmm.005447 [eCite] [Details] Citations: Scopus - 144Web of Science - 134 | |
2009 | Journal Article | Cook AL, Chen W, Thurber AE, Smit DJ, Smith AG, et al., 'Analysis of cultured human melanocytes based on polymorphisms within the SLC45A2/MATP, SLC24A5/NCKX5 and OCA2/P loci', The Journal of Investigative Dermatology: An International Journal for Research in Cutaneous Biology, 129, (2) pp. 392-405. ISSN 0022-202X (2009) [Refereed Article] DOI: 10.1038/jid.2008.211 [eCite] [Details] Citations: Scopus - 85Web of Science - 77 | |
2003 | Journal Article | Cook AL, Donatien PD, Smith AG, Murphy M, Jones MK, et al., 'Human Melanoblasts in Culture: Expression of BRN2 and Synergistic Regulation by Fibroblast Growth Factor-2, Stem Cell Factor and Endothelin-3', The Journal of Investigative Dermatology: An International Journal for Research in Cutaneous Biology, 121, (5) pp. 1150-1159. ISSN 0022-202X (2003) [Refereed Article] DOI: 10.1046/j.1523-1747.2003.12562.x [eCite] [Details] Citations: Scopus - 82Web of Science - 72 |
Journal Article
(46 outputs)Year | Citation | Altmetrics |
---|---|---|
2021 | Hernandez D, Rooney LA, Daniszewski M, Gulluyan L, Liang HH, et al., 'Culture variabilities of human iPSC-derived cerebral organoids are a major Issue for the modelling of phenotypes observed in Alzheimer's Disease', Stem Cell Reviews and Reports ISSN 2629-3277 (2021) [Refereed Article] DOI: 10.1007/s12015-021-10147-5 [eCite] [Details] Citations: Scopus - 8Web of Science - 8 Co-authors: Hewitt AW | |
2021 | Mehta A, Lu P, Taylor BVM, Charlesworth J, Cook AL, et al., 'Generation of MNZTASi001-A, a human pluripotent stem cell line from a person with primary progressive multiple sclerosis', Stem Cell Research, 57 pp. 1-4. ISSN 1873-5061 (2021) [Refereed Article] DOI: 10.1016/j.scr.2021.102568 [eCite] [Details] Citations: Scopus - 1Web of Science - 1 Co-authors: Mehta A; Taylor BVM; Charlesworth J; Burdon KP; Hewitt AW; Young KM | |
2021 | Neavin D, Nguyen Q, Daniszewsk MS, Liang HH, Chiu HS, et al., 'Single cell eQTL analysis identifies cell type-specific genetic control of gene expression in fibroblasts and reprogrammed induced pluripotent stem cells', Genome Biology, 22 Article 76. ISSN 1474-760X (2021) [Refereed Article] DOI: 10.1186/s13059-021-02293-3 [eCite] [Details] Citations: Scopus - 6Web of Science - 5 Co-authors: Vickers JC; Hewitt AW | |
2021 | Nguyen Tran MP, KC R, Patterson FM, Liu G-S, Cook AL, et al., 'Approaches for the sensitive detection of rare base and prime editing events', Methods ISSN 1046-2023 (2021) [Refereed Article] DOI: 10.1016/j.ymeth.2021.01.006 [eCite] [Details] Citations: Scopus - 1Web of Science - 1 Co-authors: Nguyen Tran MP; KC R; Patterson FM; Liu G-S; Hewitt AW | |
2021 | Stellon D, Nguyen Tran MP, Talbot J, Chear S, Mohd Khalid MKN, et al., 'CRISPR/Cas-Mediated Knock-in of genetically encoded fluorescent biosensors into the AAVS1 locus of human-induced pluripotent stem cells', Methods in Molecular Biology Article online ahead of print. ISSN 1064-3745 (2021) [Refereed Article] DOI: 10.1007/7651_2021_422 [eCite] [Details] Co-authors: Stellon D; Nguyen Tran MP; Talbot J; Chear S; Vickers JC; King AE; Hewitt AW | |
2021 | Talbot J, Chear S, Phipps A, Pebay A, Hewitt AW, et al., 'Image-based quantitation of kainic acid-induced excitotoxicity as a model of neurodegeneration in Human iPSC-Derived Neurons', Methods in Molecular Biology ISSN 1064-3745 (2021) [Refereed Article] DOI: 10.1007/7651_2021_421 [eCite] [Details] Co-authors: Talbot J; Chear S; Phipps A; Hewitt AW; Vickers JC; King AE | |
2021 | Wang Q, Chear S, Wing K, Stellon D, Tran MTN, et al., 'Use of CRISPR/Cas ribonucleoproteins for high throughput gene editing of induced pluripotent stem cells', Methods, 194 pp. 18-29. ISSN 1046-2023 (2021) [Refereed Article] DOI: 10.1016/j.ymeth.2021.02.009 [eCite] [Details] Citations: Scopus - 1Web of Science - 1 Co-authors: Chear S; Wing K; Stellon D; Talbot J; Hewitt AW | |
2020 | Li F, Wing K, Wang J-H, Luu CD, Bender JA, et al., 'Comparison of CRISPR/Cas endonucleases for in vivo retinal gene editing', Frontiers in Cellular Neuroscience, 14 Article 570917. ISSN 1662-5102 (2020) [Refereed Article] DOI: 10.3389/fncel.2020.570917 [eCite] [Details] Citations: Scopus - 5Web of Science - 5 Co-authors: Wing K; Bender JA; Lu Q; Nguyen Tran MP; Young KM; Liu G-S; Hewitt AW | |
2020 | Munoz SS, Engel M, Balez R, Do-Ha D, Cabral-da-Silva MC, et al., 'A simple differentiation protocol for generation of induced pluripotent stem cell-derived basal forebrain-like cholinergic neurons for Alzheimer's disease and frontotemporal dementia disease modeling', Cells, 9, (9) Article E2018. ISSN 2073-4409 (2020) [Refereed Article] DOI: 10.3390/cells9092018 [eCite] [Details] Citations: Scopus - 10Web of Science - 9 Co-authors: Hewitt AW | |
2020 | Ooi L, Dottori M, Cook AL, Engel M, Gautam V, et al., 'If human brain organoids are the answer to understanding dementia, what are the questions?', The Neuroscientist, 26, (5-6) pp. 438-454. ISSN 1073-8584 (2020) [Refereed Article] DOI: 10.1177/1073858420912404 [eCite] [Details] Citations: Scopus - 8Web of Science - 7 Co-authors: King AE | |
2019 | Li F, Hung SSC, Mohd Khalid MKN, Wang J-H, Chrysostomou V, et al., 'Utility of self-destructing CRISPR/Cas constructs for targeted gene editing in the retina', Human Gene Therapy, 30, (11) pp. 1349-1360. ISSN 1043-0342 (2019) [Refereed Article] DOI: 10.1089/hum.2019.021 [eCite] [Details] Citations: Scopus - 12 Co-authors: Singh V; Wing K; Bender JA; King AE; Hewitt AW; Liu G-S | |
2019 | Nguyen Tran MP, Mohd Khalid MKN, Pebay A, Cook AL, Liang HH, et al., 'Screening of CRISPR/Cas base editors to target the AMD high-risk Y402H complement factor H variant', Molecular Vision, 25 pp. 174-182. ISSN 1090-0535 (2019) [Refereed Article] PMID: 30996586 [eCite] [Details] Citations: Web of Science - 6 Co-authors: Nguyen Tran MP; Liu G-S; Hewitt AW | |
2019 | Ranson N, Veldhuis M, Mitchell B, Fanning S, Cook AL, et al., 'NLRP3-dependent and -independent processing of interleukin (IL)-1β in active ulcerative colitis', International Journal of Molecular Sciences, 20, (1) pp. 1-15. ISSN 1422-0067 (2019) [Refereed Article] DOI: 10.3390/ijms20010057 [eCite] [Details] Citations: Scopus - 33Web of Science - 33 Co-authors: Ranson N; Kunde D; Eri R | |
2018 | Ashworth Briggs EL, Toh T, Eri R, Hewitt AW, Cook AL, 'Uteroglobin and FLRG concentrations in aqueous humor are associated with age in primary open angle glaucoma patients', BMC Ophthalmology, 18, (1) pp. 1-8. ISSN 1471-2415 (2018) [Refereed Article] DOI: 10.1186/s12886-018-0723-4 [eCite] [Details] Citations: Scopus - 4Web of Science - 2 Co-authors: Ashworth Briggs EL; Eri R; Hewitt AW | |
2018 | Ranson N, Veldhuis M, Mitchell B, Fanning S, Cook AL, et al., 'Nod-like receptor pyrin-containing protein 6 (NLRP6) is up-regulated in ileal Crohn's disease and differentially expressed in goblet cells', Cellular and Molecular Gastroenterology and Hepatology, 6, (1) pp. 110-112. ISSN 2352-345X (2018) [Refereed Article] DOI: 10.1016/j.jcmgh.2018.03.001 [eCite] [Details] Citations: Scopus - 14Web of Science - 16 Co-authors: Ranson N; Kunde D; Eri R | |
2016 | Gill KP, Hung SSC, Sharov A, Lo CY, Needham K, et al., 'Enriched retinal ganglion cells derived from human embryonic stem cells', Scientific Reports, 6 Article 30552. ISSN 2045-2322 (2016) [Refereed Article] DOI: 10.1038/srep30552 [eCite] [Details] Citations: Scopus - 57Web of Science - 54 Co-authors: Hewitt AW | |
2016 | Hewitt AW, Cook AL, Pebay A, 'Peeking into the molecular trove of discarded surgical specimens', Clinical and Experimental Ophthalmology, 44, (8) pp. 661-662. ISSN 1442-6404 (2016) [Letter or Note in Journal] DOI: 10.1111/ceo.12837 [eCite] [Details] Co-authors: Hewitt AW | |
2016 | McCaughey T, Chen CY, De Smit E, Rees G, Fenwick E, et al., 'Participant understanding and recall of informed consent for induced pluripotent stem cell biobanking', Cell and Tissue Banking, 17, (3) pp. 449-456. ISSN 1389-9333 (2016) [Refereed Article] DOI: 10.1007/s10561-016-9563-8 [eCite] [Details] Citations: Scopus - 14Web of Science - 13 Co-authors: Mackey DA; Hewitt AW | |
2016 | Murtaza M, Shan J, Matigian N, Todorovic M, Cook AL, et al., 'Rotenone susceptibility phenotype in olfactory derived patient cells as a model of idiopathic Parkinson's disease', PloS One, 11, (4) Article e0154544. ISSN 1932-6203 (2016) [Refereed Article] DOI: 10.1371/journal.pone.0154544 [eCite] [Details] Citations: Scopus - 7Web of Science - 8 | |
2016 | Randall-Demllo S, Fernando R, Brain T, Sohal SS, Cook AL, et al., 'Characterisation of colonic dysplasia-like epithelial atypia in murine colitis', World Journal of Gastroenterology, 22, (37) pp. 8334-8348. ISSN 1007-9327 (2016) [Refereed Article] DOI: 10.3748/wjg.v22.i37.8334 [eCite] [Details] Citations: Scopus - 7Web of Science - 7 Co-authors: Randall-Demllo S; Sohal SS; Guven N; Kunde D; Eri R | |
2015 | Ashworth Briggs EL, Toh TY, Eri RD, Hewitt A, Cook AL, 'TIMP1, TIMP2, and TIMP4 are increased in aqueous humor from primary open angle glaucoma patients', Molecular Vision, 21 pp. 1162-1172. ISSN 1090-0535 (2015) [Refereed Article] PMID: 26539028 [eCite] [Details] Citations: Web of Science - 31 Co-authors: Ashworth Briggs EL; Eri RD; Hewitt A | |
2015 | Cook AL, Snow ET, Binns HA, Cook PS, 'Self-reported Student Confidence in Troubleshooting Ability Increases After Completion of an Inquiry-Based PCR Practical', Biochemistry and Molecular Biology Education, 43, (5) pp. 316-323. ISSN 1470-8175 (2015) [Refereed Article] DOI: 10.1002/bmb.20881 [eCite] [Details] Citations: Scopus - 5Web of Science - 5 Co-authors: Snow ET; Binns HA; Cook PS | |
2014 | Eri R, Cook A, Brown N, 'Reflections on the value of mapping the final theory examination in a molecular biochemistry unit', Journal of Microbiology and Biology Education, 15, (1) pp. 53-54. ISSN 1935-7885 (2014) [Refereed Article] DOI: 10.1128/jmbe.v15i1.679 [eCite] [Details] Co-authors: Eri R; Brown N | |
2014 | Herbert KJ, Cook AL, Snow ET, 'SIRT1 modulates miRNA processing defects in p53-mutated human keratinocytes', Journal of Dermatological Science, 74, (2) pp. 142-149. ISSN 0923-1811 (2014) [Refereed Article] DOI: 10.1016/j.jdermsci.2014.01.008 [eCite] [Details] Citations: Scopus - 11Web of Science - 11 Co-authors: Herbert KJ; Snow ET | |
2014 | Herbert KJ, Cook AL, Snow ET, 'SIRT1 inhibition restores apoptotic sensitivity in p53-mutated human keratinocytes', Toxicology and Applied Pharmacology, 277, (3) pp. 288-297. ISSN 0041-008X (2014) [Refereed Article] DOI: 10.1016/j.taap.2014.04.001 [eCite] [Details] Citations: Scopus - 16Web of Science - 16 Co-authors: Herbert KJ; Snow ET | |
2014 | Herbert KJ, Holloway A, Cook AL, Chin PS, Snow ET, 'Arsenic exposure disrupts epigenetic regulation of SIRT1 in human keratinocytes', Toxicology and Applied Pharmacology, 281, (1) pp. 136-145. ISSN 0041-008X (2014) [Refereed Article] DOI: 10.1016/j.taap.2014.09.012 [eCite] [Details] Citations: Scopus - 27Web of Science - 20 Co-authors: Herbert KJ; Holloway A; Chin PS; Snow ET | |
2013 | Osmond-McLeod MJ, McLeod RIW, Oytam Y, McCall MJ, Feltis B, et al., 'Surface coatings of ZnO nanoparticles mitigate differentially a host of transcriptional, protein and signalling responses in primary human olfactory cells', Particle and Fibre Toxicology, 10, (54) pp. 1-18. ISSN 1743-8977 (2013) [Refereed Article] DOI: 10.1186/1743-8977-10-54 [eCite] [Details] Citations: Scopus - 29Web of Science - 26 | |
2013 | Shabala L, Walker EJ, Eklund A, Randall-Demllo S, Shabala S, et al., 'Exposure of colonic epithelial cells to oxidative and endoplasmic reticulum stress causes rapid potassium efflux and calcium influx', Cell Biochemistry and Function, 31, (7) pp. 603-611. ISSN 1099-0844 (2013) [Refereed Article] DOI: 10.1002/cbf.2946 [eCite] [Details] Citations: Scopus - 7Web of Science - 7 Co-authors: Shabala L; Walker EJ; Randall-Demllo S; Shabala S; Guven N; Eri RD | |
2011 | Beaumont KA, Hamilton NA, Moores MT, Cairncross O, Cook AL, et al., 'The Recycling Endosome Protein Rab17 Regulates Melanocytic Filopodia Formation and Melanosome Trafficking', Traffic: The International Journal of Intracellular Transport, 12, (5) pp. 627-43. ISSN 1398-9219 (2011) [Refereed Article] DOI: 10.1111/j.1600-0854.2011.01172.x [eCite] [Details] Citations: Scopus - 65Web of Science - 63 | |
2011 | Boyle GM, Woods SL, Bonazzi VF, Stark MS, Hacker E, et al., 'Melanoma cell invasiveness is regulated by miR-211 suppression of the BRN2 transcription factor', Pigment Cell & Melanoma Research, 24, (3) pp. 525-537. ISSN 1755-148X (2011) [Refereed Article] DOI: 10.1111/j.1755-148X.2011.00849.x [eCite] [Details] Citations: Scopus - 133Web of Science - 126 | |
2011 | Cook AL, Vitale AM, Ravishankar S, Matigian N, Sutherland GT, et al., 'NRF2 Activation Restores Disease Related Metabolic Deficiencies in Olfactory Neurosphere-Derived Cells from Patients with Sporadic Parkinson's Disease', PLoS ONE, 6, (7) Article E21907. ISSN 1932-6203 (2011) [Refereed Article] DOI: 10.1371/journal.pone.0021907 [eCite] [Details] Citations: Scopus - 67Web of Science - 65 | |
2010 | Matigian N, Abrahamsen G, Sutharsan R, Cook AL, Vitale AM, et al., 'Disease-specific, neurosphere-derived cells as models for brain disorders', Disease models & mechanisms, 3, (11) pp. 785-798. ISSN 1754-8403 (2010) [Refereed Article] DOI: 10.1242/dmm.005447 [eCite] [Details] Citations: Scopus - 144Web of Science - 134 | |
2010 | Stark MS, Tyagi S, Nancarrow DJ, Boyle GM, Cook AL, et al., 'Characterization of the Melanoma miRNAome by Deep Sequencing', PLoS One, 5:e9685, (3) ISSN 1932-6203 (2010) [Refereed Article] DOI: 10.1371/journal.pone.0009685 [eCite] [Details] Citations: Scopus - 172Web of Science - 161 | |
2009 | Cook AL, Chen W, Thurber AE, Smit DJ, Smith AG, et al., 'Analysis of cultured human melanocytes based on polymorphisms within the SLC45A2/MATP, SLC24A5/NCKX5 and OCA2/P loci', The Journal of Investigative Dermatology: An International Journal for Research in Cutaneous Biology, 129, (2) pp. 392-405. ISSN 0022-202X (2009) [Refereed Article] DOI: 10.1038/jid.2008.211 [eCite] [Details] Citations: Scopus - 85Web of Science - 77 | |
2009 | Flammiger A, Besch R, Cook AL, Maier T, Sturm RA, et al., 'SOX9 and SOX10 but Not BRN2 Are Required for Nestin Expression in Human Melanoma Cells', The Journal of Investigative Dermatology: An International Journal for Research in Cutaneous Biology, 129, (4) pp. 945-953. ISSN 0022-202X (2009) [Refereed Article] DOI: 10.1038/jid.2008.316 [eCite] [Details] Citations: Scopus - 40Web of Science - 39 | |
2008 | Beaumont KA, Shekar SN, Cook AL, Duffy DL, Sturm RA, 'Red hair is the null phenotype of MC1R', Human Mutation, 29, (8) pp. E88-E94. ISSN 1098-1004 (2008) [Refereed Article] DOI: 10.1002/humu.20788 [eCite] [Details] Citations: Scopus - 63 | |
2008 | Smith AG, Beaumont KA, Smit DJ, Thurber AE, Cook AL, et al., 'PPAR gamma agonists attenuate proliferation and modulate Wnt/a-catenin signalling in melanoma cells', International Journal of Biochemistry and Cell Biology, 41, (4) pp. 844-852. ISSN 1357-2725 (2008) [Refereed Article] DOI: 10.1016/j.biocel.2008.08.037 [eCite] [Details] Citations: Scopus - 30Web of Science - 23 | |
2007 | Newton RL, Cook AL, Roberts DW, Leonard JH, Sturm RA, 'Post-transcriptional regulation of melanin biosynthetic enzymes by cAMP and resveratrol in human melanocytes', Journal of Investigative Dermatology, 127, (9) pp. 2216-2227. ISSN 0022-202X (2007) [Refereed Article] DOI: 10.1038/sj.jid.5700840 [eCite] [Details] Citations: Scopus - 89Web of Science - 82 | |
2005 | Cook AL, Smith AG, Smit DJ, Leonard JH, Sturm RA, 'Co-expression of SOX9 and SOX10 during melanocytic differentiation in vitro. Experimental Cell Research', Experimental Cell Research, 308, (1) pp. 222-235. ISSN 0014-4827 (2005) [Refereed Article] DOI: 10.1016/j.yexcr.2005.04.019 [eCite] [Details] Citations: Scopus - 57Web of Science - 54 | |
2004 | Ven Gele M, Boyle GM, Cook AL, Vandesompele J, Boonefaes T, et al., 'Gene expression profiling reveals distinct expression patterns for Classic versus Variant Merkel cell phenotypes and new classifier genes to distinguish Merkel cell from small cell lung carcinoma', Oncogene, 23, (15) pp. 2732-2742. ISSN 0950-9232 (2004) [Refereed Article] DOI: 10.1038/sj.onc.1207421 [eCite] [Details] Citations: Scopus - 54Web of Science - 43 | |
2003 | Cook AL, Donatien PD, Smith AG, Murphy M, Jones MK, et al., 'Human Melanoblasts in Culture: Expression of BRN2 and Synergistic Regulation by Fibroblast Growth Factor-2, Stem Cell Factor and Endothelin-3', The Journal of Investigative Dermatology: An International Journal for Research in Cutaneous Biology, 121, (5) pp. 1150-1159. ISSN 0022-202X (2003) [Refereed Article] DOI: 10.1046/j.1523-1747.2003.12562.x [eCite] [Details] Citations: Scopus - 82Web of Science - 72 | |
2003 | Leonard JH, Marks LH, Chen W, Cook AL, Boyle GM, et al., 'Screening of human primary melanocytes of defined melanocortin-1 receptor genotype: pigmentation marker, ultrastructural and UV-survival studies', Pigment Cell & Melanoma Research, 16, (3) pp. 198-207. ISSN 1755-148X (2003) [Refereed Article] DOI: 10.1034/j.1600-0749.2003.00033.x [eCite] [Details] Citations: Scopus - 37Web of Science - 36 | |
2002 | Leonard JH, Cook AL, Van Gele M, Boyle GM, Inglis KJ, et al., 'Proneural and Proneuroendocrine Transcription Factor Expression In Cutaneous Mechanoreceptor (Merkel) Cells and Merkel Cell Carcinoma', International Journal of Cancer, 101, (2) pp. 103-110. ISSN 1097-0215 (2002) [Refereed Article] DOI: 10.1002/ijc.10554 [eCite] [Details] Citations: Scopus - 67Web of Science - 67 | |
2001 | Cook AL, Pollock PM, Welch J, Walsh MD, Bowman RV, et al., 'CDKN2A is not the principle target of deletions on the short arm of chromosome 9 in neuroendocrine (Merkel cell) carcinoma of the skin', International Journal of Cancer, 93, (3) pp. 361-367. ISSN 1097-0215 (2001) [Refereed Article] DOI: 10.1002/ijc.1352 [eCite] [Details] Citations: Scopus - 11Web of Science - 10 | |
2001 | Van Gele M, Leonard JH, Van Roy N, Cook AL, De Paepe A, et al., 'Frequent allelic loss at 10q23 but low incidence of PTEN mutations in Merkel cell carcinoma', International Journal of Cancer, 92, (3) pp. 409-413. ISSN 1097-0215 (2001) [Refereed Article] DOI: 10.1002/ijc.1209 [eCite] [Details] Citations: Scopus - 53Web of Science - 49 | |
2000 | Leonard JH, Cook AL, Nancarrow D, Hayward N, Van Gele M, et al., 'Deletion mapping on the short arm of chromosome 1 in Merkel cell carcinoma', Cancer Detection and Prevention, 24, (6) pp. 620-627. ISSN 0361-090X (2000) [Refereed Article] Citations: Web of Science - 26 |
Chapter in Book
(3 outputs)Year | Citation | Altmetrics |
---|---|---|
2005 | Cook AL, Boyle GM, Leonard JH, Parsons PG, Sturm RA, 'BRN2 in melanocytic cell development, differentiation and transformation', From Melanocytes to Melanoma: The Progression of Malignancy, Humana Press Inc, Hearing, VJ, & Leong, APL (ed), Tolowa, New Jersey, pp. 149-168. ISBN 1-58829-459-5 (2005) [Research Book Chapter] | |
2003 | Leonard JH, Cook AL, Van Gele M, Speleman F, Sturm RA, 'Expression of developmentally regulated transcription factors in Merkel cell carcinoma', The Merkel Cell Structure-Development-Function-Cancerogenesis, Springer-Verlag, Bauman, KI, Halata, Z & Moll, I (ed), Germany, pp. 203-218. ISBN 978-3-642-05574-4 (2003) [Research Book Chapter] | |
2003 | Van Gele M, Boyle GM, Cook AL, Boonefaes T, Rottiers P, et al., 'Gene expression profiling reveals two distinct subtypes of Merkel Cell Carcinoma', In: The Merkel Cell Structure-Development-Function-Cancerogensesis, Springer-Verlag, Bauman, KI, Halata, Z & Moll, I (ed), Germany, pp. 195-202. ISBN 978-3-642-05574-4 (2003) [Research Book Chapter] |
Review
(1 outputs)Year | Citation | Altmetrics |
---|---|---|
2008 | Cook AL, Sturm RA, 'POU domain transcription factors: BRN2 as a regulator of melanocytic growth and tumourigenesis', Pigment Cell and Melanoma Research, 21, (6) pp. 611-626. (2008) [Substantial Review] |
Conference Publication
(4 outputs)Year | Citation | Altmetrics |
---|---|---|
2014 | Cook AL, 'Defining conditions to direct human pluripotent stem cells towards the glaucoma-affected trabecular meshwork cell lineage', Australian Health & Medical Research Congress, 16-19 November, 2014, Melbourne, Australia (2014) [Conference Extract] | |
2014 | Cook AL, Ashworth Briggs EL, Toh T, Eri RD, Hewitt A, 'Defining conditions to direct human pluripotent stem cells towards the glaucoma-affected trabecular meshwork cell lineage', 2014 Australian Health and Medical Research Congress (AHMRC), 16-19 November, 2014, Melbourne, Australia (2014) [Conference Extract] Co-authors: Ashworth Briggs EL; Eri RD; Hewitt A | |
2014 | Keith LJ, Briggs ELA, Davidson KC, Wong RCB, Pebay A, et al., 'Defining conditions to direct human pluripotent stem cells towards the glaucoma-affected trabecular meshwork cell lineage', Narrowing the Gap Between Stem Cell Science and Cell Therapy, 9-11 November, 2014, Victoria, Australia (2014) [Conference Extract] Co-authors: Keith LJ; Hewitt A | |
2012 | Cook AL, Walker EJ, Shan J, Matigian N, Wells CA, et al., 'Nrf2 dysfunction in patient-derived cell lines in a new model of sporadic Parkinsons disease', Proceedings of Keystone Symposium in Molecular and Cellular Biology, 22-27 October 2012, Tokyo, Japan (2012) [Conference Extract] Co-authors: Walker EJ |
Other Public Output
(1 outputs)Year | Citation | Altmetrics |
---|---|---|
2015 | Cook AL, 'Researchers see hope for glaucoma sufferers using stem cells', The Mercury, The Mercury, AUGUST 17, 2015 (2015) [Media Interview] |
Grants & Funding
Dr Cook has extensive research experience as a lead or co-investigator on past and current grants from NHMRC, Clifford Craig Medical Research Trust, Cancer Council Tasmania, Cancer Council Queensland, Brain Foundation, and the University of Tasmania.
Funding Summary
Number of grants
32
Total funding
Projects
- Description
- We will use our induced pluripotent stem cell (iPSC) resource to break Batten disease down into discrete cellular contexts of the BBB endothelial cells, pericytes, and astrocytes to provide the advances in knowledge that can guide mechanism-based hypotheses regarding new treatment strategies.
- Funding
- Batten Disease Support and Research Association Australia ($55,000)
- Scheme
- Grant
- Administered By
- University of Tasmania
- Research Team
- Cook AL; Sutherland BA
- Year
- 2022
- Description
- Axons are long processes that allow for communication between our nerve cells and muscles to enable movement. During MND, nerve cell axons degenerate, leading to loss of motor function and mortality; the mechanism of which we do not understand. In this project, we will use human iPSC-derived nerve cells to investigate what pathways are involved in axon degeneration through omics approaches, including RNA-seq and proteomics. By understanding why axons are vulnerable, and what pathways dysfunction during MND, we can design novel therapies to prevent and treat axon degeneration in MND.
- Funding
- Motor Neurone Disease Research Australia ($99,491)
- Scheme
- Grant - Innovator
- Administered By
- University of Tasmania
- Research Team
- Phipps AJ; Atkinson RAK; Perry SE; King AE; Cook AL
- Year
- 2022
- Description
- To advance therapeutic strategies for Batten disease, we will use our existing isogenic iPSC models with CLN3 variants to:Aim 1: Measure CLN3-dependent changes in neuronal network activity using multielectrode arrays.Aim 2: Quantify CLN3-dependent changes in lysosomal, mitochondrial and autophagy function in neurons.Aim 3: Identify CNS-penetrant drugs that exhibit efficacy against CLN3-variant associated neuron changes.
- Funding
- Batten Disease Support and Research Association ($49,400)
- Scheme
- Grant-Research
- Administered By
- University of Tasmania
- Research Team
- Cook AL; Hewitt A; King AE; Perry SE
- Year
- 2021
- Description
- The critical importance of understanding how the brain functions is evidenced by international investment in thisarea of research. For example, in the US, BRAIN (Brain Research through Advancing InnovativeNeurotechnologies) has been supported with $1-3 billion, while in Europe the Human Brain Project is supportedwith $1.5 billion. This proposal falls directly within the scope of these with its focus on new platform technology tounderpin fundamental molecular studies of neuronal circuits. It is in the national interest of Australia to undertakeinternationally leading cross-disciplinary research in this area, a position confirmed in the Academy of Sciencecoordinated Think Tank on Inspiring smarter brain research in Australia. Being the first to develop this newplatform technology, will allow Australian researchers to be at the forefront of determining the mechanismsunderlying neurodegenerative diseases, and the development of new treatments. In addition, direct financialbenefit could be obtained through the technology, for example, through licensing novel microchamber designs.
- Funding
- Australian Research Council ($545,563)
- Scheme
- Grant-Discovery Projects
- Administered By
- University of Tasmania
- Research Team
- Dickson TC; King AE; Cook AL
- Period
- 2020 - 2022
- Grant Reference
- DP200103193
- Description
- Loss of axonal connections between nerve cells results in reduced cognitive function and memory loss. Axons, like cells, can orchestrate their own death mechanisms and the SARM1 protein mediates Wallerian degeneration the best known axon death pathway. It is not yet understood, why neurons have evolved to have this axon degeneration pathway; whether it is aberrantly activate in neurodegenerative disease or whether it protects the nervous system from adverse nerve cell connections. By using a combination of methods, including axon injury and neurodegeneration mouse models, and human stem cell research, we will identify the exact role of SARM1 in the response to injury and neurodegeneration and determine whether blocking axons from this protein is beneficial or harmful. Our team is comprised of world leaders in investigating axon pathology, degeneration and regeneration in neurodegenerative disease and injury, and outcomes of this research will advance treatment of diseases such as dementia and motor-neuron disease.
- Funding
- National Health & Medical Research Council ($597,651)
- Scheme
- Grant-Ideas
- Administered By
- University of Tasmania
- Research Team
- King AE; Canty A; Cook AL; Perry SE
- Period
- 2020 - 2022
- Grant Reference
- 1187156
- Description
- Our overarching hypothesis is that TDP-43 mislocalisation leads to altered axonal and exosomal protein expression, and which may underpin the mechanism of TDP-43 pathological spread leading to neurodegeneration of motor circuits in ALS. To begin testing this hypothesis, we have designed experiments to address three Specific Aims:Aim 1: To characterize human iPS cell-based models of TDP-43 mislocalisationAim 2: To quantify proteins differentially expressed in axons and exosomes of iPS cell-derived neurons with and without TDP-43 mislocalisationAim 3: To quantify transmission of TDP-43 mislocalisation and altered phosphorylation in a human cerebral organoid model
- Funding
- Motor Neurone Disease Research Australia ($99,665)
- Scheme
- Grant - Innovator
- Administered By
- University of Tasmania
- Research Team
- Cook AL; King AE; Atkinson RAK; Perry SE; Hewitt A
- Year
- 2019
- Description
- iPSCs can be differentiated into multiple cell types, including neurons and pericytes. We wish to differentiate iPSCs from the same parental lineage into pericytes, a vascular cell with roles in blood-brain barrier maintenance and regulation of cerebral blood flow. The interaction between pericytes and neurons has not been extensively studied, and these models will allow the determination of how these cells interact leading to novel research with relevance to understanding normal brain function and how this contributes to neurodegenerative diseases.
- Funding
- University of Tasmania ($9,980)
- Scheme
- Grant-Research Enhancement Program
- Administered By
- University of Tasmania
- Research Team
- Cook AL; Sutherland BA
- Year
- 2019
- Description
- The overriding hypothesis of this work is that Clustered Regularly Interspersed Short Palindromic Repeat (CRISPR) and CRISPR-associated (Cas) technology can be used as an anticipatory cure to well-defined inherited retinal diseases caused by single nucleotide substitutions. The aim of this proposal is to use patient-specific induced pluripotent stem cells (iPSCs) to demonstrate the safety and efficacy of CRISPR/Cas-base editing constructs for the anticipatory cure of Usher Syndrome. In preparation for a Phase 1, first in human clinical trial, we will construct and test single vector plasmids to deliver SaCas9 with an appropriate promoter, single guided RNA (sgRNA) and an optimised adenine base editor (ABE) to correct the Arg245Ter PCDH15 disease causing variant.
- Funding
- Medical Research Future Fund ($733,314)
- Scheme
- Grant - Accelerated Research
- Administered By
- University of Tasmania
- Research Team
- Hewitt A; Pebay A; Cook AL
- Year
- 2019
- Description
- We will test an HDAC6 inhibitor in models of ALS. We will investigate the HDA6 inhibitor activity of novel compounds
- Funding
- FightMND ($997,046)
- Scheme
- Grant - Drug Development Grant
- Administered By
- University of Tasmania
- Research Team
- King AE; Cook AL; Guven N; Van Den Bosch L; Dickson TC; Blizzard C; Vickers JC; Smith JA; Alty JE; Leung JY; Perry SE
- Period
- 2019 - 2022
- Description
- The aim of this project is to generate and characterize an isogenic control line from a famifia) AD iPSC line using CRISPR/Cas gene editing. This will yield a powerful tool to study impacts of intracellular AD processes on stress signaling and for future studies of AD pathology at the WickingCentre.
- Funding
- University of Tasmania ($20,000)
- Scheme
- Grant-Research Enhancement Program
- Administered By
- University of Tasmania
- Research Team
- Sinclair D; Cook AL
- Year
- 2019
- Description
- This proposal is for strategic and specialised equipment underpinned by basic infrastructure required tosupport neurodegenerative disease research within the CHM.
- Funding
- University of Tasmania ($1,700,000)
- Scheme
- null
- Administered By
- University of Tasmania
- Research Team
- Hewitt A; Cook AL; Dickson TC
- Year
- 2019
- Description
- The goal of this research is to find which genes and proteins are responsible for our abilities to form memories, understand language and make decisions. By using stem cells from many different people, we will make nerve cells in the laboratory and study how these nerve cells function. We can than relate our results from the laboratory to the ability of our study participants to form memories, understand language and make decisions. The genes and proteins we identify in this way may indicate new ways to delay the onset or progression of dementia in people.
- Funding
- University of Tasmania ($24,443)
- Scheme
- Grant- Research Enhancement Program
- Administered By
- University of Tasmania
- Research Team
- Cook AL
- Year
- 2018
- Description
- Batten disease is a rare childhood disease that results in dementia and a progressive loss of vision, and which can be due to mutation of the CLN3 gene. Using advances in stem cell technologies, we will study how the Batten disease-causing mutations in CLN3 differently affect nerve health.
- Funding
- Royal Hobart Hospital Research Foundation ($24,898)
- Scheme
- Grant-Establishment
- Administered By
- University of Tasmania
- Research Team
- Cook AL; Hewitt A; King AE; Ware T
- Year
- 2017
- Description
- Batten disease is a rare childhood disease that results in dementia and a progressive loss of vision, and which can be due to mutation of the several genes, including CLN2 and CLN3. Using advances in stem cell technologies, we will study how the Batten disease-causing mutations in these genes differently affect nerve health.
- Funding
- Batten Disease Support and Research Association ($51,411)
- Scheme
- Grant-Research
- Administered By
- University of Tasmania
- Research Team
- Cook AL; Hewitt A; King AE; Pebay A; Grubman A
- Year
- 2017
- Description
- The CRISPR/Cas system, used by bacteria to counter viral intrusion, can edit DNA in specific sites. The application of this technology opens the very real prospect of anticipatory cures to well-defined inherited retinal diseases and we propose to expand the pre-clinical investigation of DNA editing of cells in the retina.
- Funding
- Royal Hobart Hospital Research Foundation ($25,000)
- Scheme
- Grant-Establishment
- Administered By
- University of Tasmania
- Research Team
- Hewitt A; Liu R; Cook AL
- Year
- 2017
- Description
- We will apply leading genomic techniques to identify the genes essential for the proliferation of the Tasmanian Devil Facial Tumour. By systematically disrupting each gene in the cancer, we will also identify genes which help the tumour evade the immune system.
- Funding
- University of Tasmania Foundation Inc ($33,500)
- Scheme
- Grant-Dr Eric Guiler Tasmanian Devil Research Gran
- Administered By
- University of Tasmania
- Research Team
- Hewitt A; Liu G; Woods GM; Flies AS; Cook AL
- Year
- 2017
- Description
- Our understanding of many cancers has improved dramatically over the past decade predominantly due to our ability to sequence entire genomes, at scale. Yet, we still require a better understanding of the underlying mechanisms that initiate and perpetuate cancers, as well as gene-based factors that initiate the transition from indolent to aggressive cancers with a propensity to metastasize. CRISPR/Cas is proving a robust, powerful and necessary tool in the laboratory that will undoubtedly underpin the next breakthrough in the field of cancer. As such, it is essential that we develop this capability at the University of Tasmania in a timely manner.The specific AIMS of this proposal are to:Aim 1: Establish CRISPR/Cas screening at the University of Tasmania as a tool to identify genesthat drive aggressive and metastatic cancers.Aim 2: Perform a genome-wide CRISPR/Cas negative selection (loss-of-function) screen toidentify genes essential for proliferation and survival, as well as metastatic behaviour.
- Funding
- University of Tasmania Foundation Inc ($63,845)
- Scheme
- Grant-Cancer Research
- Administered By
- University of Tasmania
- Research Team
- Taberlay PC; Hewitt A; Holloway AF; Liu G; Dickinson JL; Brettingham-Moore KH; Fitzgerald L; Eri RD; Cook AL
- Year
- 2017
- Description
- Glaucoma is the leading cause of irreversible blindness worldwide and there is currently no definitive treatment for this common disease. We have recently identified a number of genes associated with glaucoma, and we now seek to use this new genetic insight to test potentialdrug targets. This work will provide data that will lead directly to pre-clinical testing of FDAapproved drugs, and which may ultimately identify novel treatments for this devastating disease.
- Funding
- Ophthalmic Research Institute of Australia ($50,000)
- Scheme
- Grant-Research
- Administered By
- University of Tasmania
- Research Team
- Cook AL; Hewitt A
- Year
- 2016
- Description
- Investigate the efficacy of single Clustered Regularly Interspersed Short Palindromic Repeat (CRISPR) and CRISPR-associated (Cas) plasmids for gene editing in the retina.Validate our novel self-destructing CRISPR/Cas system and ensure, after initial activity, no functional CRISPR/Cas protein is produced.
- Funding
- Bayer Pharma AG ($72,634)
- Scheme
- Award-Global Ophthalmology Awards
- Administered By
- University of Tasmania
- Research Team
- Hewitt A; Cook AL
- Period
- 2016 - 2017
- Funding
- Clifford Craig Foundation ($71,000)
- Scheme
- Grant
- Administered By
- University of Tasmania
- Research Team
- Cook AL; Hewitt A; Pebay A; Keith LJ
- Year
- 2015
- Description
- The aims of this project are to: 1) define the entirety of genes expressed within the trabecular meshwork samples of glaucoma patients and controls; 2) correlate glaucoma-specific trabecular meshwork gene expression signatures to clinical parameters.
- Funding
- Clifford Craig Foundation ($36,300)
- Scheme
- Grant
- Administered By
- University of Tasmania
- Research Team
- Cook AL; Toh TY; Kunde DA; Hewitt A
- Year
- 2015
- Description
- Glaucoma is a degenerative disease attributed to progressive loss of neurons within the optic nerve. There is no cure for this disease, with treatment strategies predominantly focused on reducing intraocular pressure (IOP), which remains the only modifiable risk factor. IOP is generally considered to be due to inhibited outflow of aqueous humor, the fluid within the eye, through a specialised tissue known as the trabecular meshwork. The trabecular meshwork (TM) is highly fibrous, consisting of several layers of cells overlying extracellular matrix (ECM) structures. During normal ageing, increased ECM deposition occurs in the deeper layers of the trabecular meshwork, and this appears to be increased in glaucoma. It is not known how this occurs, but it has been speculated that changes in expression and activity of ECM remodelling proteins, typified by matrix metalloproteinases (MMPs) and their regulators, tissue inhibitor of metalloproteinases (TIMPs), may be responsible. Several studies have examined gene expression and the ECM structure in normal and glaucomatous trabecular meshwork, as well as the presence of MMPs and TIMPS in aqueous humor, but no study has attempted to correlate the extent of ECM deposition with MMP or TIMP activity using trabecular meshwork tissue and aqueous humor from the same patient.
- Funding
- Clifford Craig Foundation ($36,235)
- Scheme
- Grant
- Administered By
- University of Tasmania
- Research Team
- Cook AL; Toh TY; Hewitt A; Eri RD
- Year
- 2014
- Funding
- University of Tasmania ($14,500)
- Scheme
- Grant-Research Enhancement (REGS)
- Administered By
- University of Tasmania
- Research Team
- Cook AL
- Year
- 2014
- Description
- The aims of the project are: to study the expression levels of different inflammation molecules and inflammasome components in ulcerative colities patients, UC-associated-colorectal patients, sporadic colorectal patients compared to controls; to identify the cell populations where different inflammasome components are localised.
- Funding
- Clifford Craig Foundation ($24,409)
- Scheme
- Grant
- Administered By
- University of Tasmania
- Research Team
- Eri RD; Lloyd D; Mitchell B; Fanning S; Ahuja KDK; Cook AL
- Year
- 2014
- Funding
- Clifford Craig Foundation ($27,273)
- Scheme
- Grant
- Administered By
- University of Tasmania
- Research Team
- Cook AL; Eri RD; Hewitt A; Toh TY
- Year
- 2013
- Funding
- Clifford Craig Foundation ($54,546)
- Scheme
- Grant
- Administered By
- University of Tasmania
- Research Team
- Eri RD; Cook AL; Mitchell B; Wilson R
- Period
- 2013 - 2014
- Funding
- Australian Rotary Health Research Fund ($54,000)
- Scheme
- Scholarship-Funding Partners
- Administered By
- University of Tasmania
- Research Team
- Eri RD; Spring K; Cook AL
- Period
- 2013 - 2015
- Description
- The project aims to establish and bank glaucoma patient-specific fibroblast cell lines and conduct molecular analyses on these cell lines.
- Funding
- Centre for Eye Research Aust Ltd ($10,000)
- Scheme
- Contract Research
- Administered By
- University of Tasmania
- Research Team
- Cook AL; Eri RD
- Year
- 2012
- Funding
- University of Tasmania ($1,300)
- Scheme
- Grant-Conference Support Scheme
- Administered By
- University of Tasmania
- Research Team
- Cook AL
- Year
- 2012
- Funding
- Cancer Council of Tasmania ($22,300)
- Scheme
- Grant-Small
- Administered By
- University of Tasmania
- Research Team
- Cook AL; Snow ET; Holloway AF
- Year
- 2012
- Funding
- University of Tasmania ($15,277)
- Scheme
- Grant-Institutional Research Scheme
- Administered By
- University of Tasmania
- Research Team
- Cook AL
- Year
- 2011
- Funding
- Brain Foundation ($22,000)
- Scheme
- Grant-Research
- Administered By
- University of Tasmania
- Research Team
- Cook AL
- Year
- 2010
Research Supervision
Dr Cook has successfully supervised one PhD student to completion (now at Oxford University, UK) as well as a Masters and several Honours students. He currently supervises several PhD candidates both in a primary and co-supervisory capacity. Dr Cook is interested in hearing from high performing prospective students eager to gain experience in stem cell-based approaches to modelling of neurodegenerative diseases.
Current
9
Completed
9
Current
Degree | Title | Commenced |
---|---|---|
PhD | Patient-specific Stem Cell Models of Batten Disease | 2018 |
PhD | Genome-Wide Analysis Of Gene Regulatory Networks In Primary Open Angle Glaucoma | 2018 |
PhD | Stem Cell Models of Dementia Risk and Related Neurodegenerative Diseases | 2019 |
PhD | How Does Cerebral Amyloid Angiopathy Contribute Towards Vascular and Pericyte Dysfunction in Alzheimer's Disease? | 2019 |
PhD | Engineering and Evolving Molecular Cas-based Therapies | 2020 |
PhD | Developing Drugs to Protect Neuronal Connections in Neurodegenerative Diseases | 2020 |
PhD | Investigating Stress-related Therapeutic Targets for Alzheimer's Disease in Human Neuronal Cells | 2020 |
PhD | Investigating the Role of SARM1 in Axon Degeneration using Human Induced Pluripotent Stem Cells | 2021 |
PhD | Stem Cell Models of The Blood-brain Barrier | 2022 |
Completed
Degree | Title | Completed |
---|---|---|
PhD | Harnessing Trabecular Meshwork Cells for the Molecular Profiling of Primary Open-angle Glaucoma Candidate: Qinyi Lu | 2020 |
PhD | Applying Bioinformatic Tools to Better Understand Eye Diseases Candidate: Vikrant Singh | 2020 |
PhD | A CRISPR Way to Treat Usher Syndrome Candidate: Qi Wang | 2020 |
PhD | Development of CRISPR/Cas-Mediated Gene Editing in the Retina Candidate: Fan Li | 2020 |
PhD | Aqueous Humour Protein Imbalances in Primary Open-angle Glaucoma and Their Effects on Trabecular Meshwork Cell Function Candidate: Esther Lara Ashworth Briggs | 2018 |
PhD | Inflammasome Activity in the Inflammatory Bowel Diseases Candidate: Nicole Maree Ranson | 2018 |
PhD | Genetic Ablation of CCR6 Confers Differential Exacerbation in a Spontaneous Colitis Model Candidate: Waheedha Fathima Basheer | 2017 |
PhD | Investigation of Neoplastic Transition of the Colonic Epithelium in the Winnie Mouse Model of Chronic Colitis Candidate: Sarron Yael Randall-Demllo | 2017 |
PhD | Epigenetic mechanisms of arsenic-induced transformation in human keratinocytes Candidate: Katharine Jane Herbert | 2014 |