Profiles

Owen Marshall

UTAS Home Dr Owen Marshall

Owen Marshall

Senior Research Fellow

Hobart CBD Campuses

+61 3 6226 4248 (phone)

owen.marshall@utas.edu.au

View more on Dr Owen Marshall in WARP

Fields of Research

  • Biochemistry and cell biology (310199)
  • Epigenetics (incl. genome methylation and epigenomics) (310504)
  • Central nervous system (320903)
  • Cellular nervous system (320902)
  • Developmental genetics (incl. sex determination) (310503)
  • Genome structure and regulation (310508)
  • Cell development, proliferation and death (310102)
  • Systems biology (310114)
  • Regenerative medicine (incl. stem cells) (320606)
  • Autonomic nervous system (320901)
  • Neurology and neuromuscular diseases (320905)
  • Gene expression (incl. microarray and other genome-wide approaches) (310505)
  • Genomics (310509)
  • Neurogenetics (310511)
  • Cancer cell biology (321101)
  • Proteomics and intermolecular interactions (excl. medical proteomics) (310109)
  • Animal cell and molecular biology (310902)
  • Beverage chemistry and beverage sensory science (300601)

Research Objectives

  • Expanding knowledge in the health sciences (280112)
  • Clinical health (200199)
  • Expanding knowledge in the biological sciences (280102)
  • Expanding knowledge in the biomedical and clinical sciences (280103)
  • Diagnosis of human diseases and conditions (200101)
  • Other health (209999)
  • Treatment of human diseases and conditions (200105)
  • Prevention of human diseases and conditions (200104)
  • Neonatal and child health (200506)
  • Expanding knowledge in the mathematical sciences (280118)
  • Control of pests, diseases and exotic species in terrestrial environments (180602)

Publications

Total publications

22

Journal Article

(20 outputs)
YearCitationAltmetrics
2021Aughey GN, Delandre C, McMullen JPD, Southall TD, Marshall OJ, 'FlyORF-TaDa allows rapid generation of new lines for in vivo cell-type-specific profiling of protein-DNA interactions in Drosophila melanogaster', G3: Genes, Genomes, Genetics, 11, (1) pp. 1-6. ISSN 2160-1836 (2021) [Refereed Article]

DOI: 10.1093/g3journal/jkaa005 [eCite] [Details]

Citations: Web of Science - 1

Co-authors: Delandre C; McMullen JPD

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2021Diaz-Torres A, Rosales-Nieves AE, Pearson JR, Santa-Cruz Mateos C, Marin-Menguiano M, et al., 'Stem cell niche organization in the Drosophila ovary requires the ECM component Perlecan', Current Biology, 31, (8) pp. 1744-1753.e5. ISSN 0960-9822 (2021) [Refereed Article]

DOI: 10.1016/j.cub.2021.01.071 [eCite] [Details]

Citations: Scopus - 4Web of Science - 3

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2021Hatch HAM, Belalcazar HM, Marshall OJ, Secombe J, 'A KDM5-Prospero transcriptional axis functions during early neurodevelopment to regulate mushroom body formation', Elife, 10 pp. 1-29. ISSN 2050-084X (2021) [Refereed Article]

DOI: 10.7554/eLife.63886 [eCite] [Details]

Citations: Scopus - 3Web of Science - 3

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2019Delandre C, Marshall OJ, 'United colours of chromatin? Developmental genome organisation in flies', Biochemical Society Transactions, 47, (2) pp. 691-700. ISSN 0300-5127 (2019) [Refereed Article]

DOI: 10.1042/BST20180605 [eCite] [Details]

Co-authors: Delandre C

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2019Gervais L, van den Beek M, Josserand M, Salle J, Stefanutti M, et al., 'Stem cell proliferation is kept in check by the chromatin regulators Kismet/CHD7/CHD8 and Trr/MLL3/4', Developmental Cell, 49, (4) pp. 556-573. ISSN 1534-5807 (2019) [Refereed Article]

DOI: 10.1016/j.devcel.2019.04.033 [eCite] [Details]

Citations: Scopus - 12Web of Science - 12

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2018Doupe DP, Marshall OJ, Dayton H, Brand AH, Perrimon N, 'Drosophila intestinal stem and progenitor cells are major sources and regulators of homeostatic niche signals', Proceedings of the National Academy of Sciences of The United States of America pp. 1-6. ISSN 0027-8424 (2018) [Refereed Article]

DOI: 10.1073/pnas.1719169115 [eCite] [Details]

Citations: Scopus - 30Web of Science - 30

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2017Marshall OJ, Brand AH, 'Chromatin state changes during neural development revealed by in vivo cell-type specific profiling', Nature Communications, 8, (1) Article 2271. ISSN 2041-1723 (2017) [Refereed Article]

DOI: 10.1038/s41467-017-02385-4 [eCite] [Details]

Citations: Scopus - 35Web of Science - 35

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2016Marshall OJ, Brand AH, 'damidseq_pipeline: an automated pipeline for processing DamID sequencing datasets', Bioinformatics, 31, (20) pp. 3371-3373. ISSN 1367-4803 (2016) [Refereed Article]

DOI: 10.1093/bioinformatics/btv386 [eCite] [Details]

Citations: Scopus - 56Web of Science - 52

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2016Marshall OJ, Southall TD, Cheetham SW, Brand AH, 'Cell-type-specific profiling of protein-DNA interactions without cell isolation using targeted DamID with next-generation sequencing', Nature Protocols, 11, (9) pp. 1586-1598. ISSN 1754-2189 (2016) [Refereed Article]

DOI: 10.1038/nprot.2016.084 [eCite] [Details]

Citations: Scopus - 55Web of Science - 52

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2014Garsed DW, Marshall OJ, Corbin VD, Hsu A, Di Stefano L, et al., 'The architecture and evolution of cancer neochromosomes', Cancer Cell, 26, (5) pp. 653-667. ISSN 1535-6108 (2014) [Refereed Article]

DOI: 10.1016/j.ccell.2014.09.010 [eCite] [Details]

Citations: Scopus - 108Web of Science - 102

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2013Southall TD, Gold KS, Egger B, Davidson CM, Caygill EE, et al., 'Cell-type-specific profiling of gene expression and chromatin binding without cell isolation: assaying RNA Pol II occupancy in neural stem cells', Developmental Cell, 26, (1) pp. 101-112. ISSN 1534-5807 (2013) [Refereed Article]

DOI: 10.1016/j.devcel.2013.05.020 [eCite] [Details]

Citations: Scopus - 119Web of Science - 118

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2012Chan FL, Marshall OJ, Saffery R, Kim BW, Earle E, et al., 'Active transcription and essential role of RNA polymerase II at the centromere during mitosis', Proceedings of the National Academy of Sciences of The United States of America, 109, (6) pp. 1979-1984. ISSN 0027-8424 (2012) [Refereed Article]

DOI: 10.1073/pnas.1108705109 [eCite] [Details]

Citations: Scopus - 167Web of Science - 173

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2012Green LC, Kalitsis P, Chang TM, Cipetic M, Kim H, et al., 'Contrasting roles of condensin I and condensin II in mitotic chromosome formation', Journal of Cell Science, 125, (6) pp. 1591-1604. ISSN 0021-9533 (2012) [Refereed Article]

DOI: 10.1242/jcs.097790 [eCite] [Details]

Citations: Scopus - 113Web of Science - 113

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2012Marshall OJ, Choo KHA, 'Putative CENP-B paralogues are not present at mammalian centromeres', Chromosoma, 121, (12) pp. 169-179. ISSN 0009-5915 (2012) [Refereed Article]

DOI: 10.1007/s00412-011-0348-3 [eCite] [Details]

Citations: Scopus - 5Web of Science - 6

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2009Marshall OJ, Choo KHA, 'Neocentromeres come of age', PL o S Genetics, 5, (3) Article e1000370. ISSN 1553-7390 (2009) [Contribution to Refereed Journal]

DOI: 10.1371/journal.pgen.1000370 [eCite] [Details]

Citations: Scopus - 9Web of Science - 8

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2008Marshall OJ, Chueh AC, Wong LH, Choo KHA, 'Neocentromeres: new insights into centromere structure, disease development, and karyotype evolution', American Journal of Human Genetics, 82, (2) pp. 261-282. ISSN 0002-9297 (2008) [Refereed Article]

DOI: 10.1016/j.ajhg.2007.11.009. [eCite] [Details]

Citations: Scopus - 271Web of Science - 276

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2008Marshall OJ, Marshall AT, Choo KHA, 'Three-dimensional localization of CENP-A suggests a complex higher order structure of centromeric chromatin', Journal of Cell Biology, 183, (7) pp. 1193-1202. ISSN 0021-9525 (2008) [Refereed Article]

DOI: 10.1083/jcb.200804078 [eCite] [Details]

Citations: Scopus - 44Web of Science - 45

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2004Marshall OJ, 'PerlPrimer: cross-platform, graphical primer design for standard, bisulphite and real-time PCR', Bioinformatics, 20, (15) pp. 2471-2472. ISSN 1367-4803 (2004) [Contribution to Refereed Journal]

DOI: 10.1093/bioinformatics/bth254 [eCite] [Details]

Citations: Scopus - 476Web of Science - 468

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2001Marshall OJ, Harley VR, 'Identification of an interaction between SOX9 and HSP70', F E B S Letters: (Federation of European Biochemical Societies), 496, (2-3) pp. 75-80. ISSN 0014-5793 (2001) [Refereed Article]

DOI: 10.1016/S0014-5793(01)02407-3 [eCite] [Details]

Citations: Scopus - 29Web of Science - 27

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2000Marshall OJ, Harley VR, 'Molecular mechanisms of SOX9 action', Molecular Genetics and Metabolism, 71, (3) pp. 455-462. ISSN 1096-7192 (2000) [Refereed Article]

DOI: 10.1006/mgme.2000.3081 [eCite] [Details]

Citations: Scopus - 42Web of Science - 39

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Chapter in Book

(2 outputs)
YearCitationAltmetrics
2008Marshall OJ, Choo KHA, 'Neocentromeres', The Kinetochore: From Molecular Discoveries to Cancer Therapy, Springer-Verlag New York, P De Wulf and WC Earnshaw (ed), New York City, pp. 77-106. ISBN 978-1-4419-2398-1 (2008) [Research Book Chapter]

DOI: 10.1007/978-0-387-69076-6 [eCite] [Details]

Citations: Scopus - 4

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2007Marshall OJ, 'Graphical Design of Primers with PerlPrimer', PCR Primer Design. Methods in Molecular Biology, Humana Press, A Yuryev (ed), Totowa, NJ, pp. 403-414. ISBN 978-1-58829-725-9 (2007) [Research Book Chapter]

DOI: 10.1007/978-1-59745-528-2_21 [eCite] [Details]

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Grants & Funding

Funding Summary

Number of grants

5

Total funding

$1,639,704

Projects

There and back again: understanding the epigenetic remodelling that turns glia into glioma (2020 - 2022)$536,847
Description
Glia are a major cell type within the brain that protect, nourish and insulate neurons. Like neurons, glia are formed via differentiation from neural stem cells. Although differentiation is typically associated with epigenetic locks to prevent a reversal of cell fate, some glia can overcome these inhibitions and differentiate to form glioma tumours capable of rapid proliferation and resistance to treatment.Understanding how glioma cells manage to break out of their chromatin environment jail represents a key means to understanding these tumours and to combating their resistance and lethality. However, the epigenetic changes that occur during glial differentiation and glioma de-differentiation remain a mystery.Here, using the fruit fly as a model organism, together with our new cell-type specific Chromatin TaDa system, we will profile the epigenetic configuration of glial cells within the brain. In order to understand glial differentiation, we will profile the binding of the key cell fate determinant that triggers gliogenesis, Gcm, together with two key epigenetic factors that drive this change, Cbp and the SAGA complex. Finally we will use a well-characterised and relevant fly model of glioma to understand the pathway of differentiation and tumourogenesis. By using of variants of this model to represent different stages of malignancy we will track the epigenetic changes involved in cancer formation. Together with a rapid RNAi screen for chromatin modifying complexes, we will identify the key epigenetic changes to target in interventions. These predictions will be tested on a panel of human glioblastoma cell lines.Our team represents an international collaboration uniquely placed to tackle this problem, consisting of an expert in chromatin states and cell-type specific profiling (CI Marshall), an expert in glia and glial cell fate (CI Giangrande) and experts in glioma and glioma models (AIs Casas Tinto, Quinn and Parsons).
Funding
National Health & Medical Research Council ($536,847)
Scheme
Grant-Ideas
Administered By
University of Tasmania
Research Team
Marshall O; Giangrande A
Period
2020 - 2022
Grant Reference
1185220
Chromatin State Changes in Intestinal Stem Cell Ageing (2019)$35,000
Description
Adult stem cells must divide to produce new cells to renew and repair tissues. A failure to produce enoughcells could lead to tissue failure while an overproduction of cells can lead to compromised tissue functionand cancer. The incidence of colorectal cancer increases with age, suggesting that in ageing intestinal stemcells this balance may be lost. We use the fruit fly gut as a simple model to study intestinal stem cells. Manyof the signals and genes that regulate human intestinal stem cells are conserved to fruit flies. In youngadult flies the stem cells divide to maintain the gut, but in ageing flies they become mis-regulated andover-proliferate.Changes in cell behaviour such as increased stem cell proliferation usually occur through changes in geneexpression. DNA is packaged into structures called chromatin, some forms of chromatin allow geneexpression, while others are repressive, causing genes to be switched off. This means that chromatin stateplays an important role in stem cell regulation. Individual chromatin components have been shown toregulate stem cells but how chromatin state contributes to stem cell ageing is not known. This project willcharacterize the changes in chromatin states that occur when intestinal stem cells age. This will improveour understanding of how adult stem cells become mis-regulated with age; a critical process for health anddisease. Our work will identify new mechanisms of regulation that could be targeted to improve tissuefunction with age.
Funding
The Royal Society ($35,000)
Scheme
Grant
Administered By
Durham University
Research Team
Doupe D; Marshall O
Year
2019
Confocal Microscopy (2019)$370,000
Description
Confocal microscope. There is an urgent need for additional confocal microscopes in the Medical Science Precinct research laboratory. By way of example, there is a 2 month waiting list for new users to be trained to use microscopes and a longer waiting period to gain access to image experiments.
Funding
University of Tasmania ($370,000)
Scheme
null
Administered By
University of Tasmania
Research Team
Marshall O; Young K
Year
2019
Imaging to overcome dementia (2018)$150,000
Description
This grant will enable the purchase of a high-end confocal microscope that will dramatically increase imaging capacity at the Menzies Institute for Medical Research, University of Tasmania. A large number of research groups based at the Menzies are conducting research into the causes of dementia and other neurological diseases, and these will strongly benefit from the acquisition of the new microscope. The microscope will increase both the productivity and impact of the research of these groups, and also enable increased collaborative links between Menzies researchers and national and international partners
Funding
Ian Potter Foundation ($150,000)
Scheme
Grant - Medical Research
Administered By
University of Tasmania
Research Team
Marshall O
Year
2018
A genome-wide analysis of the epigenetic control of learning and memory (2017 - 2019)$547,857
Description
The ability to form and recall memories is a fundamental function of the brain. This ability is affected by neurodegenerative diseases such as Alzheimer's Disease. This project will uncover the regulatory framework that defines the brain cells involved in forming memories, and how these change during Alzheimer's Disease progression.
Funding
National Health & Medical Research Council ($547,857)
Scheme
Grant-Project
Administered By
University of Tasmania
Research Team
Marshall O; Dickson TC; Southall T
Period
2017 - 2019
Grant Reference
1128784

Research Supervision

Current

4

Completed

1

Current

DegreeTitleCommenced
PhDTranscriptional Control of Gene Networks in Living Neurons2017
PhDDeveloping Systems to Optogenetically Inhibit Neuropeptide Exocytosis2018
PhDInvestigating the Epigenetic Remodelling Events that Produce Glia and Glioma2021
PhDHow Does Diet Affect Transcriptional and Epigenetic Changes Occurring During Ageing and Neurodegeneration?2021

Completed

DegreeTitleCompleted
PhDDevelopment of Optogenetic Approaches to Selectively Modulate G Protein Signalling
Candidate: Jayde Louise Lockyer
2021