Cell-specific proteomics of organoids

Cell type-specific proteomics of brain organoid models of lysosomal storage disorder

Degree type

PhD

Closing date

27 March 2023

Campus

Hobart

Citizenship requirement

Domestic/International

About the research project

Generation of cell-type specific data from stem cell-derived brain organoids is limited to RNAseq (e.g. scRNA-seq), or to technologies that require extensive isolation of different cell populations using FACS. There is currently limited data about the proteome of particular cell types from organoids models because they require isolation through FACS. Here we propose an alternative approach that involves direct metabolic labelling of proteins as they are translated in a manner that enables ‘click chemistry’ pull down and proteomics. We will edit the endogenous alleles of canonical cell type marker genes, such that methionine-tRNA synthetase (MarsL274G) is expressed in a cell type-specific manner.  This project is initially focused on technology development, and will then apply the newly developed technology to investigate cell-autonomous and non cell-autonomous contributions to neurodegenerative diseases with a particular emphasis on lysosomal storage disorders. Applicants with interests in gene editing, stem cell biology, and bioinformatics/proteomics are encouraged to apply.

Primary Supervisor

Meet A/Prof Tony Cook

Funding

Applicants will be considered for a Research Training Program (RTP) scholarship or Tasmania Graduate Research Scholarship (TGRS) which, if successful, provides:

  • a living allowance stipend of $31,500 per annum (2023 rate, indexed annually) for 3.5 years
  • a relocation allowance of up to $2,000
  • a tuition fees offset covering the cost of tuition fees for up to four years (domestic applicants only)

If successful, international applicants will receive a University of Tasmania Fees Offset for up to four years.

As part of the application process you may indicate if you do not wish to be considered for scholarship funding.

Eligibility

Applicants should review the Higher Degree by Research minimum entry requirements.

Additional eligibility criteria specific to this project/scholarship:

  • Applications are open to Domestic/ International/ Onshore applicants
  • Applications are open to applications from neuroscience/cell biology/molecular biology disciplines or similar background only
  • Applicants must be able to undertake the project on-campus

Selection Criteria

The project is competitively assessed and awarded.  Selection is based on academic merit and suitability to the project as determined by the College.

Additional essential selection criteria specific to this project:

  • Strong communication skills
  • Strong record keeping skills
  • Familiar with PC2 laboratory research

Additional desirable selection criteria specific to this project:

  • Experience in contemporary cell-based techniques, particularly iPSC culture and differentiation, and/or CRISPR/Cas gene editing

Application process

There is a three-step application process:

  1. Select your project, and check you meet the eligibility and selection criteria;
  2. Contact the Primary Supervisor, A/Prof Tony Cook to discuss your suitability and the project's requirements; and
  3. Submit an application by the closing date listed above.
    • Copy and paste the title of the project from this advertisement into your application. If you don’t correctly do this your application may be rejected.
    • As part of your application, you will be required to submit a covering letter, a CV including 2 x referees and your project research proposal.

Following the application closing date applications will be assessed within the College. Applicants should expect to receive notification of the outcome by email by the advertised outcome date.

Apply now Explore other projects

Why the University of Tasmania?

Worldwide reputation for research excellence

Quality supervision and support

Tasmania offers a unique study lifestyle experience